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Detection Mini

Licence

Copyright © M Goillandeau IMN / CNRS Université de Bordeaux (2012,2013)

[michel.goillandeau at u-bordeaux.fr]

This software is a computer program whose purpose is to detect the presence of mini action potential during an electrophysiological recording with the patch-clamp method (mEPSCs, eIPSCs)..

This software is governed by the CeCILL-C license under French law and
abiding by the rules of distribution of free software. You can use,
modify and/ or redistribute the software under the terms of the CeCILL-C license as circulated by CEA, CNRS and INRIA at the following URL
"http://www.cecill.info".

As a counterpart to the access to the source code and rights to copy,
modify and redistribute granted by the license, users are provided only
with a limited warranty and the software’s author, the holder of the
economic rights, and the successive licensors have only limited
liability.

In this respect, the user’s attention is drawn to the risks associated
with loading, using, modifying and/or developing or reproducing the
software by the user in light of its specific status of free software,
that may mean that it is complicated to manipulate, and that also
therefore means that it is reserved for developers and experienced
professionals having in-depth computer knowledge. Users are therefore
encouraged to load and test the software’s suitability as regards their
requirements in conditions enabling the security of their systems and/or
data to be ensured and, more generally, to use and operate it in the
same conditions as regards security.

The fact that you are presently reading this means that you have had
knowledge of the CeCILL-C license and that you accept its terms.
CeCILL-C license (txt)

i) License key
To send the update, i want to know the people and computers that use my program.
If you have a file with invalid key license, the program is limited.
The file result is restricted to 50 events.
Install the software and click to Help:License Key and send an email to the author with your ID and Computer number.

ii) Acknowledgment
If you write a paper using this program, I would be very happy that i am in Acknowledgment with the name of this program name and the author.

Software

This program efficiently detects the presence of mini action potential during an electrophysiological recording with the patch-clamp method (mEPSCs, eIPSCs).
For the moment, it is compatible with files created by ABF pClamp (© Molecular Devices).Version 9 and 10 are supported.

The characteristics of PA are exported to .csv spreadsheet format
Column

  1. Num: Event number in the list of events detected.
    You may have jumps corresponding to remove event.
  2. Positions: Time of the peak.
  3. Max-BL: Peak amplitude (measured on the biological signal but not on fit signal)
  4. Baseline: Value of the baseline before the event.
  5. Rise time: Duration between the 10 and 90% of the peak amplitude
  6. Offset Exp: Value of the baseline of fit signal
  7. Amplitude Exp: Value of the amplitude of fit signal
  8. Tau exp: tau of exponential
  9. R*R exp: R2, square of Pearson correlation coefficient
  10. Higner 5* tau: 1 if the next event is over 5*tau (n), the fit is not disturbed by this event.
  11. Higher 5*Tau_Exp(n-1): 1 if the previous event is over 5*tau (n-1), the amplitude and rise time are not disturbed by this event.
  12. And operation:
    i) 1 or 3 if the next event is over 5*tau (n)
    ii) 2 or 3 if the previous event is over 5*tau (n-1)
    iii) 3 if the event preceding is over 5*tau (n-1) and the next was over than 5*tau

You can export to spreadsheet file or/and image files(jpeg).

  • Amplitude vs Tau
  • Amplitude vs Rise Time
  • Rise Time vs Tau
  • Average trace
  • FFT

Manual

Manual (.pdf)

Download

Download (.zip)

References

1- Noradrenergic Modulation of Intrinsic and Synaptic Properties of Lumbar Motoneurons in the Neonatal Rat Spinal Cord.
Tartas M, Morin F, Barrière G, Goillandeau M, Lacaille JC, Cazalets JR, Bertrand SS.
Front Neural Circuits. 2010 Mar 8

2- Altered pallido-pallidal synaptic transmission leads to aberrant firing of globus pallidus neurons in a rat model of Parkinson’s disease.
Miguelez C, Morin S, Martinez A, Goillandeau M, Bezard E, Bioulac B, Baufreton J.
J Physiol. 2012 Nov 15

3- Dopamine-dependent long-term depression at subthalamo-nigral synapses is lost in experimental parkinsonism.
Dupuis JP1, Feyder M, Miguelez C, Garcia L, Morin S, Choquet D, Hosy E, Bezard E, Fisone G, Bioulac BH, Baufreton J.
J Neurosci. 2013 Sep 4;33(36):14331-41. doi: 10.1523/JNEUROSCI.1681-13.2013.

4- Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission.
Murphy-Royal C, Dupuis JP, Varela JA, Panatier A, Pinson B, Baufreton J, Groc L, Oliet SHR.
Nature Neurosci. 2015 18(2):219-26.